"[We] will oppose any legislation that will result in nearly a million job losses," Democratic Alliance spokesperson Sej Motau said.
Banning labour brokers would result in over 850 000 people, who are currently employed by labour brokers, losing their jobs.
Motau said that during deliberations on a draft amendment bill in Parliament on Wednesday, ANC MPs had pushed for amendments that would effectively ban labour brokers.
On Thursday, the ANC said reports that its MPs would oppose parts of the draft Labour Relations Amendment Bill dealing with labour brokers were "distorted".
"The bill was presented to parliament by the minister of labour after extensive deliberations by social partners at Nedlac [the National Economic Development and Labour Council]," ANC chief whip Mathole Motshekga said in a statement.
"We dismiss the distortion that these provisions are fait accompli because they came from Nedlac.
"There has not been any decision by the [labour] portfolio committee on any clause in the amendment bill."
Motshekga said committee members of all parties were urged to consider views on the bill coming from the public consultation process and Nedlac.
"The ANC will avoid any push for a vote on any of the clauses, but will seek consensus on these issues with all the other political parties," he said.