"E-tolling will kill jobs in Gauteng and make it harder for people to make ends meet," Democratic Alliance spokesperson Mmusi Maimane said in a statement.
"The power of the vote is the surest way to stop tolls."
He was responding to the Supreme Court of Appeal's judgment on Wednesday dismissing the Opposition to Urban Tolling Alliance's (Outa) challenge to e-tolls.
Judge Fritz Brand refused the appeal against the SA National Roads Agency Limited's (Sanral) plans to introduce e-tolls. No costs order was made.
However, Brand said the order granted by the High Court in Pretoria directing the appellants (Outa) to pay the respondents' (Sanral) costs was set aside and replaced by a ruling that there be no costs order.
Outa chairperson Wayne Duvenage said the alliance and its lawyers were studying Brand's judgement.
Maimane said it was not the end of the road for the fight against e-tolls.
He said the DA had contributed R1m to Outa's court action because it believed it was important to contest the principle of e-tolling.